Wur library phd thesis

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MSc theses

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The model fitted well to the inactivation data of individual cultures. Variability between the different cultures of the five tested micro-organisms was observed.

Tips and tricks for PhDs - WUR

Therefore, an average value of the parameters of the individual cultures was used to predict inactivation as a function of temperature for a chosen short holding time. In Chapter 6, a systematic evaluation of the individual effects of electric field strength and pulse width in combination with heat on the inactivation of E. Senftenberg, L. A wide range of conditions has been tested, including both moderate intensity as well as high intensity PEF.

Tips and tricks for PhDs

Both electric field strength and pulse width were shown to be important for microbial inactivation. Inactivation kinetics of the tested conditions were compared to an equivalent thermal reference process, based on parameter estimates obtained in Chapter 5. A nonthermal pulse effect was observed for three specific sets of conditions in addition to the thermal effect responsible for inactivation.

The effectivity of this moderate intensity PEF condition was evaluated for E.

Interestingly, this moderate intensity PEF condition showed the same effectivity for all matrices in the pH range of 3. This suggests that a different mechanism is responsible for inactivation at moderate intensity conditions compared to high intensity conditions. Speculations on the mechanism responsible for inactivation are made in Chapter 8. No differences for pH and soluble solids were found after application of any treatment, and only small differences were observed for colour and vitamin C content after PEF and thermal treatment, mainly for the conditions applied at higher temperature.